1. 新污染物的内分泌干扰和神经毒性效应评价及分子机制研究：基于有害结局路径框架，通过活体斑马鱼模型、离体细胞模型和计算机模拟等，评价环境中广泛存在的新污染物的内分泌干扰和神经发育毒性效应，寻找分子靶点，并深入研究其分子作用机制, 为进一步开展生态风险评价提供理论依据和数据支持。

2. 水环境中污染物的暴露水平及生态风险评价：针对典型水体中多种污染物开展周期性监测，获取典型污染物对代表性水生生物的急慢性毒性数据，通过多层次风险评价方法评估其对水生态系统的潜在风险，为生态保护和环境管理政策的制定提供科学依据。

1. 国家自然科学基金委员会，面上项目，新型溴代阻燃剂对中枢神经系统的内暴露、毒性效应及分子机制研究，2022.1-2025.12，主持；

2. 国家重点研发计划，长江黄河专项子任务，流域候选特征污染物对敏感指示水生生物的毒性测试，2021.12-2025.11，主持；

3. 国家自然科学基金委员会，重点项目，新型溴代阻燃剂的水环境暴露、毒性效应及分子机制，2018.1-2022.12，参加；

4. 中国工程院应急攻关项目委托，新型冠状病毒传播与环境的关系及风险防控项目委托，2020.2.-2021.2主持；

5. 高校/企业/事业单位委托项目，武汉市重点区域地表水及底泥抗生素的含量分析，2021.4-2021.12，主持；

6. 高校/企业/事业单位委托项目，丹江口库区地表水及底泥农药及抗生素含量分析项目，2020.8-2020.12，主持；

7. 中科院水生所特色研究所服务项目子课题, 典型水域污染调查及对特有鱼类的安全评价, 2015.7.-2018.12, 主持；

9. 中国科学院先导专项B, 典型内分泌干扰物对斑马鱼发育毒性效应与分子机制研究, 2014.7-2019.6, 参加；

9. 国家自然科学基金委员会，青年基金，斑马鱼多巴胺系统作为多溴联苯醚作用靶点的毒性效应及分子机制研究，2014.1-2017.12，主持；

10. 中国科学院水生生物研究所知识创新工程领域前沿项目，PBDEs对斑马鱼的神经发育毒性效应和分子机理，2012.1-2014.12, 主持.

通过以上课题的研究，建立了涵盖活体测试、离体测试和计算机模拟等手段的毒性效应评价体系，获得了长江中上游多个河流、湖泊中重金属和有机污染物的监测数据，并评价了典型污染物对水生态系统的潜在风险。共发表学术论文50余篇，其中第一或（共同）通讯作者SCI论文20余篇，第一或通讯作者中文核心期刊6篇，参与编写专著1部（排名第二）。

近五年代表性论文：

1.          Zhu Biran, Lei Lei, Sun Yumiao, Shi Xiongjie, Fu Kaiyu, Hua Jianghuan, Martyniuk Christopher J., Han Jian*, Yang Lihua*, Zhou Bingsheng. Niclosamide exposure at environmentally relevant concentrations efficaciously inhibited the growth and disturbed the liver-gut axis of adult male zebrafish. Environmental science & technology 2022, xx: xxx-xxx.

2.          Sun Yumiao, Zhu Biran, Ling Siyuan, Yan Biao, Wang Xiulin, Jia Shuzhao, Martyniuk Christopher J., Zhang Wei, Yang Lihua*, Zhou Bingsheng*: Decabromodiphenyl ethane mainly affected the muscle contraction and reproductive endocrine system in female adult zebrafish. Environmental science & technology 2022, 56 (1): 470-479.

3.          Liu Sitian, Song Guili, Li Fan, Li Ruiwen, Chen Xiangping, Guo Yongyong, Zhou Fang, Wang Qiangwei, Yang Lihua*, Zhou Bingsheng: Bis (2-ethylhexyl)-2,3,4,5-tetrabromophthalate showed poor penetrability but increased the permeability of blood brain barrier: Evidences from in vitro and in vivo studies. Journal of Hazardous Materials 2022, 424:27386.

4.          Hu Zhichao^#, Yang Lihua^#, Han Jian, Liu Zishu, Zhao Yuxiang, Jin Yihao, Sheng Yaqi, Zhu Lizhong, Hu Baolan: Human viruses lurking in the environment activated by excessive use of COVID-19 prevention supplies. Environment international 2022, 163:107192.

5.          Wang Xiulin, Sun Yumiao, Fu Mengru, Chen Pengyu, Wang Qiangwei, Hua Jianghuan, Fu Kaiyu, Zhang Wei, Zhu Lifei, Yang Lihua*, Zhou Bingsheng: Nano-TiO2 Adsorbed Decabromodiphenyl Ethane and Changed Its Bioavailability, Biotransformation and Biotoxicity in Zebrafish Embryos/Larvae. Frontiers in Environmental Science 2022, 10.

6.          Chen Xiangping, Lei Lei, Liu Sitian, Han Jian, Li Ruiwen, Men Jun, Li Lin, Wei Lin, Sheng Yaqi, Yang Lihua*, Zhou Bingsheng, Zhu Lizhong: Occurrence and risk assessment of pharmaceuticals and personal care products (PPCPs) against COVID-19 in lakes and WWTP-river-estuary system in Wuhan, China. Science of The Total Environment 2021:148352.

7.          Li Ruiwen, Yang Lihua*, Han Jian, Zou Yu, Wang Yingcai, Feng Chenglian, Zhou Bingsheng: Early-life exposure to tris (1,3-dichloro-2-propyl) phosphate caused multigenerational neurodevelopmental toxicity in zebrafish via altering maternal thyroid hormones transfer and epigenetic modifications. Environmental pollution 2021, 285:117471.

8.          Yang Lihua, Ivantsova Emma, Souders Christopher L., Martyniuk Christopher J. *: The agrochemical S-metolachlor disrupts molecular mediators and morphology of the swim bladder: Implications for locomotor activity in zebrafish (Danio rerio). Ecotoxicology and Environmental Safety 2021, 208:111641.

9.          Peng Wei, Liu Sitian, Guo Yongyong, Yang Lihua*, Zhou Bingsheng: Embryonic exposure to pentabromobenzene inhibited the inflation of posterior swim bladder in zebrafish larvae. Environmental Pollution 2020, 259:113923.

10.      Yang Lihua, Huang Tao, Li Ruiwen, Souders Christopher, Rheingold Spencer, Tischuk Claire, Li Na, Zhou Bingsheng, Martyniuk Christopher J.*: Evaluation and comparison of the mitochondrial and developmental toxicity of three strobilurins in zebrafish embryo/larvae. Environmental Pollution 2020:116277.

11.      Yang Lihua, Zha Jinmiao*, Guo Yongyong, Zhou Bingsheng: Evaluation and mechanistic study of chlordecone-induced thyroid disruption: Based on in vivo, in vitro and in silico assays. Science of The Total Environment 2020, 716:136987.

12.      Zhu Biran*, He Wei, Hu Shengchao, Kong Ren, Yang Lihua*: The fate and oxidative stress of different sized SiO2 nanoparticles in zebrafish (Danio rerio) larvae. Chemosphere 2019, 225:705-712.

13.      Li Ruiwen, Wang Hengqi, Mi Chuang, Feng Chenglian, Zhang Ling*, Yang Lihua*, Zhou Bingsheng: The adverse effect of TCIPP and TCEP on neurodevelopment of zebrafish embryos/larvae. Chemosphere 2019, 220:811-817.

14.      Zhu Biran, Zhao Gang, Yang Lihua*, Zhou Bingsheng: Tetrabromobisphenol A caused neurodevelopmental toxicity via disrupting thyroid hormones in zebrafish larvae. Chemosphere 2018, 197:353-361.

15.      Yang Lihua, Zha Jinmiao*, Wang Zijian: Pentachlorophenol affected both reproductive and interrenal systems: In silico and in vivo evidence. Chemosphere 2017, 166:174-183.

论著：

周炳升，杨丽华，刘春生，等。《持久性有机污染物的内分泌干扰效应》2018年6月 科学出版社